Curvature elasticity and refolding of OmpA in large unilamellar vesicles

被引：42

作者：

Pocanschi, Cosmin L.

Patel, Geetika J.

Marsh, Derek ^{[1
]}

Kleinschmidt, Joerg H.

机构：

[1] Univ Konstanz, Fachbereich Biol, D-78547 Constance, Germany

[2] Max Planck Inst Biophys Chem, D-37070 Gottingen, Germany

来源：

BIOPHYSICAL JOURNAL | 2006年 / 91卷 / 08期

关键词：

D O I：

10.1529/biophysj.106.091439

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The stability of OmpA in large unilamellar vesicles of dilauroyl phosphatidylcholine was studied using different concentrations of urea. The effective energy of unfolding, as determined from refolding experiments, is greater than that for small sonicated unilamellar vesicles by an amount that is compatible with estimates of the elastic energy of highly curved vesicles. The on-rate for refolding and insertion is slower for large unilamellar vesicles than for small unilamellar vesicles, which indicates a contribution of vesicle strain also to the free energy of the transition state.

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收藏

页码：L75 / L77

页数：3

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