Radioprotectant screening for cryocrystallography

被引：39

作者：

Southworth-Davies, Robert J. ^{[1
]}

Garman, Elspeth F. ^{[1
]}

机构：

[1] Univ Oxford, Dept Biochem, Lab Mol Biophys, Oxford OX1 3QU, England

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2007年 / 14卷

关键词：

radioprotectants; radiation damage; macromolecular cryocrystallography;

D O I：

10.1107/S0909049506044177

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Radiation damage continues to present a problem to crystallographers using cryocooled protein crystals at third-generation synchrotrons. Free-radical scavengers have been suggested as a possible means of reducing the rate of this damage. The screening of a large number of potential radioprotectants was undertaken with an online microspectrophotometer using cystine and cysteine to model protein disulfide bonds and thiol groups, respectively. Oxidized alpha-lipoic acid was tested as a possible model disulfide bond. The evidence for the effectiveness of ascorbate as a radioprotectant was strengthened, and quinone, 2,2,6,6-tetramethyl-4-piperidone, and reduced dithiothreitol showed promise as radioprotectants.

引用

页码：73 / 83

页数：11

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