Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli

被引:60
作者
Eguchi, Y
Okada, T
Minagawa, S
Oshima, T
Mori, H
Yamamoto, K
Ishihama, A
Utsumi, R
机构
[1] Kinki Univ, Grad Sch Agr, Dept Biosci & Biotechnol, Nara 6318505, Japan
[2] Nara Inst Sci & Technol, Res & Educ Ctr Genet Informat, Ikoma 6300101, Japan
[3] Nippon Inst Biol Sci, Div Mol Biol, Tokyo 1900024, Japan
关键词
D O I
10.1128/JB.186.10.3006-3014.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Transcriptional analysis of a constitutively active mutant of the EvgA/EvgS two-component system of Escherichia coli resulted in enhanced expression of 13 PhoP/PhoQ-regulated genes, crcA, hemL, mgtA, ompT, phoP, phoQ, proP, rstA, rstB, slyB, ybjG, yrbL, and mgrB. This regulatory network between the two systems also occurred as a result of overproduction of the EvgA regulator; however, enhanced transcription of the phoPQ genes did not further activate expression of the PhoP/PhoQ-regulated genes. These results demonstrated signal transduction from the EvgA/EvgS system to the PhoP/PhoQ system in E. coli and also identified the genes that required the two systems for enhanced expression. This is one example of the intricate signal transduction networks that are posited to exist in E. coli.
引用
收藏
页码:3006 / 3014
页数:9
相关论文
共 36 条
[2]   STRUCTURAL AND GENETIC-ANALYSIS OF THE BVG-LOCUS IN BORDETELLA SPECIES [J].
ARICO, B ;
SCARLATO, V ;
MONACK, DM ;
FALKOW, S ;
RAPPUOLI, R .
MOLECULAR MICROBIOLOGY, 1991, 5 (10) :2481-2491
[3]   TRANSPOSITION AND FUSION OF LAC GENES TO SELECTED PROMOTERS IN ESCHERICHIA-COLI USING BACTERIOPHAGE-LAMBDA AND BACTERIOPHAGE-MU [J].
CASADABAN, MJ .
JOURNAL OF MOLECULAR BIOLOGY, 1976, 104 (03) :541-555
[4]   The phosphatase activity is the target for Mg2+ regulation of the sensor protein PhoQ in Salmonella [J].
Castelli, ME ;
Véscovi, EG ;
Soncini, FC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (30) :22948-22954
[5]  
Eguchi Y, 2003, MICROBIOL-SGM, V149, P2819, DOI 10.1099/mic.0.26460-0
[6]  
GARCIAVESCOVI E, 1997, J BIOL CHEM, V272, P1440
[7]   The pleiotropic two-component regulatory system PhoP-PhoQ [J].
Groisman, EA .
JOURNAL OF BACTERIOLOGY, 2001, 183 (06) :1835-1842
[8]   PhoP-PhoQ activates transcription of pmrAB, encoding a two-component regulatory system involved in Salmonella typhimurium antimicrobial peptide resistance [J].
Gunn, JS ;
Miller, SI .
JOURNAL OF BACTERIOLOGY, 1996, 178 (23) :6857-6864
[9]   Regulation of lipid a modifications by Salmonella typhimurium virulence genes phoP-phoQ [J].
Guo, L ;
Lim, KB ;
Gunn, JS ;
Bainbridge, B ;
Darveau, RP ;
Hackett, M ;
Miller, SI .
SCIENCE, 1997, 276 (5310) :250-253
[10]   Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12:: Identification of extracellular Mg2+-responsive promoters [J].
Kato, A ;
Tanabe, H ;
Utsumi, R .
JOURNAL OF BACTERIOLOGY, 1999, 181 (17) :5516-5520