Transgenic plants of cassava (Manihot esculenta) with resistance to Basta obtained by Agrobacterium-mediated transformation

被引：31

作者：

Sarria, R ^{[1
]}

Torres, E ^{[1
]}

Angel, F ^{[1
]}

Chavarriaga, P ^{[1
]}

Roca, WM ^{[1
]}

机构：

[1] CIAT, Biotechnol Res Unit, Cali, Colombia

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 04期

关键词：

Manihot esculenta; bar; ppt/Basta-resistance; useful transgenes;

D O I：

10.1007/s002990050737

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledons were used as source of explants. A non-disarmed Agrobacterium strain (CIAT 1182) was used to transfer the genes of interest into cassava cultivar MPer183. Greenhouse tests of resistance to Basta (Hoechst) showed three plant lines with different levels of tolerance to the herbicide, Based on Southern tests of transgenesis, the transformation efficiency was 1%. The results constitute the first report of the bar gene conferring herbicide resistance to cassava plants.

引用

页码：339 / 344

页数：6

共 22 条

[1]

ARIAS DI, 1995, CASSAVA BIOTECHNOLOG, P245

[2]

Calderon-Urrea A., 1988, Transformation of Manihot esculenta (cassava) using Agrobacterium tumefaciens and expression of the introduced foreign genes in transformed cell lines

[3]

CANN H, 1965, STAINING PROCEDURES

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