Circulating DNA and DNase activity in human blood

被引：169

作者：

Tamkovich, Svetlana N. ^{[1
]}

Cherepanova, Anna V. ^{[1
]}

Kolesnikova, Elena V. ^{[1
]}

Rykova, Elena Y. ^{[1
]}

Pyshnyi, Dmitrii V. ^{[1
]}

Vlassov, Valentin V. ^{[1
]}

Laktionov, Pavel P. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Div, Novosibirsk 630090, Russia

来源：

CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM IV | 2006年 / 1075卷

关键词：

circulating DNA; deoxyribonuclease activity; amplicon-based immunoassay; blood plasma; cancer;

D O I：

10.1196/annals.1368.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The concentration of circulating DNA (cirDNA) and deoxyribonuclease activity in blood plasma of healthy donors and patients with colon or stomach cancer were analyzed. The concentration of DNA was measured using Hoechst 33258 fluorescent assay after the isolation by the glass-milk protocol. A 1-kbp PCR product labeled with biotinylated forward and fluorescein-labeled reverse primers was used as a substrate for DNase. DNase activity was estimated from the data of immunochemical detection of the nonhydrolyzed amplicon. The average concentration of cirDNA in the plasma of healthy donors was low (34 +/- 34 ng/mL), and was accompanied with high DNase activity (0.356 +/- 0.410 U/mL). The increased concentrations of cirDNA in blood plasma of patients with colon and stomach cancer were accompanied by a decrease in DNase activity below the detection level of the assay. The data obtained demonstrate that low DNase activity in blood plasma of cancer patients can cause an increase in the concentration of cirDNA.

引用

页码：191 / 196

页数：6

共 20 条

[1] Interaction of keratin K1 with nucleic acids on the cell surface [J].

Chelobanov, BP ;

Laktionov, PP ;

Kharkova, MV ;

Rykova, EY ;

Pyshnyi, DV ;

Pyshnaya, IA ;

Marcus, K ;

Meyer, HE ;

Vlassov, VV .

BIOCHEMISTRY-MOSCOW, 2003, 68 (11) :1239-1246

[2]

CISAR LA, 1985, J LIPID RES, V26, P380

[3]

DEWEZ B, 1993, EUR J CLIN CHEM CLIN, V31, P793

[4]

ECONOMIDOUKARAOGLOU A, 1988, CANCER, V61, P1838, DOI 10.1002/1097-0142(19880501)61:9<1838::AID-CNCR2820610920>3.0.CO

[5]

2-R

[6] A soluble PC-1 circulates in human plasma: Relationship with insulin resistance and associated abnormalities [J].

Frittitta, L ;

Camastra, S ;

Baratta, R ;

Costanzo, BV ;

D'Adamo, M ;

Graci, S ;

Spampinato, D ;

Maddux, BA ;

Vigneri, R ;

Ferrannini, E ;

Trischitta, V .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1999, 84 (10) :3620-3625

[7] AN ANTIBODY CAPTURE BIOASSAY (ACB) FOR DNASE IN HUMAN SERUM SAMPLES [J].

GIBSON, UEM ;

CHEN, AB ;

BAKER, DL ;

SINICROPI, DV .

JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 155 (02) :249-256

[8]

Jahr S, 2001, CANCER RES, V61, P1659

[9] SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE [J].

LABARCA, C ;

PAIGEN, K .

ANALYTICAL BIOCHEMISTRY, 1980, 102 (02) :344-352

[10] Cell-surface-bound nucleic acids - Free and cell-surface-bound nucleic acids in blood of healthy donors and breast cancer patients [J].

Laktionov, PP ;

Tamkovich, SN ;

Rykova, EY ;

Bryzgunova, OE ;

Starikov, AV ;

Kuznetsova, NP ;

Vlassov, VV .

CIRCULATING NUCLEIC ACIDS IN PLASMA/SERUM III AND SERUM PROTEOMICS, 2004, 1022 :221-227

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