The ENCODE Blacklist: Identification of Problematic Regions of the Genome

被引：671

作者：

Amemiya, Haley M. ^{[1
,2
]}

Kundaje, Anshul ^{[3
]}

Boyle, Alan P. ^{[1
,2
,4
]}

机构：

[1] Univ Michigan, Grad Program Cellular & Mol Biol, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Computat Med & Bioinformat, Ann Arbor, MI 48109 USA

[3] Stanford Sch Med, Dept Genet, Stanford, CA 94305 USA

[4] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

CHIP-SEQ;

D O I：

10.1038/s41598-019-45839-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Functional genomics assays based on high-throughput sequencing greatly expand our ability to understand the genome. Here, we define the ENCODE blacklist- a comprehensive set of regions in the human, mouse, worm, and fly genomes that have anomalous, unstructured, or high signal in next-generation sequencing experiments independent of cell line or experiment. The removal of the ENCODE blacklist is an essential quality measure when analyzing functional genomics data.

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