Angiotensin II increases the intracellular calcium activity in podocytes of the intact glomerulus

被引:67
作者
Nitschke, R
Henger, A
Ricken, S
Gloy, J
Müller, V
Greger, R
Pavenstädt, H
机构
[1] Univ Freiburg, Div Nephrol, Dept Med, D-79106 Freiburg, Germany
[2] Univ Freiburg, Inst Physiol, D-79106 Freiburg, Germany
关键词
cytosolic calcium; intracellular calcium; antiotensin II type 1 receptor; injury; acute glomerulonephritis;
D O I
10.1046/j.1523-1755.2000.00810.x
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Background. Knowledge about biological functions of podocytes in the glomerulus is limited because of its unique anatomical location. Here we introduce a new method for measuring the intracellular calcium activity ([Ca2+](i)) in the podocyte in the intact glomerulus. Methods. With the help of fluorescence high-resolution digital imaging and a recently developed ultraviolet laser-scanning microscope, [Ca-2](i) was measured in fura-2-loaded glomeruli and single podocytes of intact microdissected rat glomeruli. Results. Angiotensin II (Ang II) increased [Ca2+](i) reversibly in a biphasic and concentration-dependent manner. In contrast to Ang II, bradykinin, thrombin, arginine vasopressin, and serotonin did not change [Ca2+](i) in the glomerulus. At reduced extracellular Ca2+ activity, Ang II released [Ca2+](i) from intracellular stores, but the second phase, corresponding to a Ca2+ influx from the extracellular space, was absent. The L-type Ca2+ channel blocker nicardipine did not influence the Ang II-mediated [Ca2+](i) increase, and an increase of the extracellular K+ concentration did not change [Ca2+]i in the glomerulus. The angrotensin II type I(AT(1)) receptor antagonist losartan inhibited the Ang II-mediated [Ca2+](i) increase. Confocal [Ca2+](i) measurements using fura-2 or fluo-3 or fluo-4 on the single cell level show that some of the Ang II-mediated [Ca2+](i) response originated from podocytes. Costaining with calcein allowed the identification of podocytes because of the characteristic morphology and location in relationship to the capillary network. Conclusions. These data suggest that podocytes in the intact glomerulus respond to Ang II with an increase of [Ca2+](i) via an AT1 receptor.
引用
收藏
页码:41 / 49
页数:9
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