Nitric oxide is associated with poor embryo quality and pregnancy outcome in in vitro fertilization cycles

被引:43
作者
Lee, TH
Wu, MY
Chen, MJ
Chao, KH
Ho, HN
Yang, YS
机构
[1] Natl Taiwan Univ Hosp, Dept Obstet & Gynecol, Coll Med, Taipei 100, Taiwan
[2] Natl Taiwan Univ, Dept Obstet & Gynecol, Coll Med, Taipei 10764, Taiwan
关键词
tubal infertility; pregnancy; nitric oxide; embryo quality; soluble Fas;
D O I
10.1016/j.fertnstert.2004.02.097
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To evaluate the association between follicular and serum nitric oxide (NO) levels and embryo development and outcome in IVF. Design: Prospective, case-control study. Setting: University hospital, tertiary medical center. Patient(s): Eighteen patients with tubal or peritoneal factor infertility and 18 female partners from couples with male factor infertility underwent controlled ovarian stimulation and IVF/intracytoplasmic sperm injection (ICSI). Intervention(s): Controlled ovarian stimulation and oocyte retrieval followed by IVF/ICSI and embryo culture. Main Outcome Measure(s): Degree of fragmentation of embryos and pregnancy rate. Result(s): Higher follicular NO levels were associated with advanced fragmentation of embryos. Follicular soluble Fas could not prevent embryo fragmentation. Higher serum NO levels were found among nonpregnant patients with tubal or peritoneal factor infertility. No elevated serum NO levels were found in the female partners from couples with male factor infertility. Conclusion(s): Up-regulation of serum NO is associated with implantation failure in patients with tubal or peritoneal factor infertility. (C) 2004 by American Society for Reproductive Medicine.
引用
收藏
页码:126 / 131
页数:6
相关论文
共 32 条
[1]   Human embryo fragmentation in vitro and its implications for pregnancy and implantation [J].
Alikani, M ;
Cohen, J ;
Tomkin, G ;
Garrisi, GJ ;
Mack, C ;
Scott, RT .
FERTILITY AND STERILITY, 1999, 71 (05) :836-842
[2]   Nitric oxide (NO) and interleukin-1β (IL-1β) in follicular fluid and their correlation with fertilization and embryo cleavage [J].
Barrionuevo, MJ ;
Schwandt, RA ;
Rao, PS ;
Graham, LB ;
Maisel, LP ;
Yeko, TR .
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2000, 44 (06) :359-364
[3]   Nitric oxide inhibits development of embryos and implantation in mice [J].
Barroso, RP ;
Osuamkpe, C ;
Nagamani, M ;
Yallampalli, C .
MOLECULAR HUMAN REPRODUCTION, 1998, 4 (05) :503-507
[4]   Adjuvant L-arginine treatment in controlled ovarian hyperstimulation: a double-blind, randomized study [J].
Battaglia, C ;
Regnani, G ;
Marsella, T ;
Facchinetti, F ;
Volpe, A ;
Venturoli, S ;
Flamigni, C .
HUMAN REPRODUCTION, 2002, 17 (03) :659-665
[5]   Adjuvant L-arginine treatment for in-vitro fertilization in poor responder patients [J].
Battaglia, C ;
Salvatori, M ;
Maxia, N ;
Petraglia, F ;
Facchinetti, F ;
Volpe, A .
HUMAN REPRODUCTION, 1999, 14 (07) :1690-1697
[6]  
Blashkiv TV, 2001, B EXP BIOL MED+, V132, P1034
[7]   Nitric oxide as a regulator in preimplantation embryo development and apoptosis [J].
Chen, HW ;
Jiang, WS ;
Tzeng, CR .
FERTILITY AND STERILITY, 2001, 75 (06) :1163-1171
[8]   PROTECTION FROM FAS-MEDIATED APOPTOSIS BY A SOLUBLE FORM OF THE FAS MOLECULE [J].
CHENG, JH ;
ZHOU, T ;
LIU, CD ;
SHAPIRO, JP ;
BRAUER, MJ ;
KIEFER, MC ;
BARR, PJ ;
MOUNTZ, JD .
SCIENCE, 1994, 263 (5154) :1759-1762
[9]   Nitric oxide as a bioregulator of apoptosis [J].
Chung, HT ;
Pae, HO ;
Choi, BM ;
Billiar, TR ;
Kim, YM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 282 (05) :1075-1079
[10]   Role of nitric oxide in implantation and menstruation [J].
Chwalisz, K ;
Garfield, RE .
HUMAN REPRODUCTION, 2000, 15 :96-111