Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius

被引：2

作者：

Bönisch, H

Schmidt, CL

Schäfer, G

Ladenstein, R

机构：

[1] Karolinska Inst, NOVUM, Dept Biosci, Ctr Struct Biochem, S-14157 Huddinge, Sweden

[2] Med Univ Lubeck, Dept Biochem, D-23538 Lubeck, Germany

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444900002456

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe-2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P6(1) or P6(5), with unit-cell parameters a = b = 80.19, c = 75.69 Angstrom. A complete data set has been collected to 1.64 Angstrom resolution at 100 K.

引用

页码：643 / 644

页数：2

共 11 条

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