Development of a cytolchrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices

被引：38

作者：

Cortina-Puig, Montserrat ^{[1
]}

Munoz-Berbel, Xavier ^{[1
]}

Rouillon, Regis ^{[1
]}

Calas-Blanchard, Carole ^{[1
]}

Marty, Jean-Louis ^{[1
]}

机构：

[1] Univ Perpignan, IMAGES EA 4218, F-66860 Perpignan, France

来源：

BIOELECTROCHEMISTRY | 2009年 / 76卷 / 1-2期

关键词：

Cytochrome c-based biosensor; Superoxide radicals; Antioxidant capacity; Orange juices; Screen-printed gold electrodes; IMMOBILIZED CYTOCHROME-C; OXIDATIVE STRESS; FREE-RADICALS; ELECTRODE; DISEASE; SYSTEMS; SENSOR; FOODS;

D O I：

10.1016/j.bioelechem.2009.04.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：76 / 80

页数：5

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