High affinity binding and allosteric regulation of Escherichia coli glycogen phosphorylase by the histidine phosphorylase protein, HPr

The histidine phosphocarrier protein (HPr) is an essential element in sugar transport by the bacterial phosphoenolpyruvate:sugar phosphotransferase system, Ligand fishing, using surface plasmon resonance, was used to show the binding of HPr to a nonphosphotransferase protein in extracts of Escherichia coli; the protein was subsequently identified as glycogen phosphorylase (GP), The high affinity (association constant similar to 10s M-1), species-specific interaction was also demonstrated in electrophoretic mobility shift experiments by polyacrylamide gel electrophoresis, Equilibrium ultracentrifugation analysis indicates that HPr allosterically regulates the oligomeric state of glycogen phosphorylase. HPr binding increases GP activity to 250% of the level in control assays, Kinetic analysis of coupled enzyme as says shows that the binding of HPr to GP causes a decrease in the K-m for glycogen and an increase in the V-max for phosphate, indicating a mixed type activation, The stimulatory effect of E, coli HPr on E, coli GP activity is species-specific, and the unphosphorylated form of HPr activates GP more than does the phosphorylated form, Replacement of specific amino acids in]HPr results in reduced GP activation; HPr residues Arg-17, Lys-24, Lys-27, Lys-40, Ser-46, Gln-51, and Lys-72 were established to be important, This novel mechanism for the regulation of GP provides the first evidence directly linking E, coli HPr to the regulation of carbohydrate metabolism.