Multiple effects of an anti-human immunodeficiency virus nucleocapsid inhibitor on virus morphology and replication

被引:33
作者
Berthoux, L [1 ]
Péchoux, C [1 ]
Darlix, JL [1 ]
机构
[1] Ecole Normale Super, INSERM, Unite Virol Humaine, LaboRetro, F-69364 Lyon 07, France
关键词
D O I
10.1128/JVI.73.12.10000-10009.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human immunodeficiency virus type 1 nucleocapsid protein is a major structural component of the virion core and a key factor involved in proviral DNA synthesis and virus formation. 2,2'-Dithiobenzamides (DIBA-1) and related compounds that are inhibitors of NCp7 are thought to eject zinc ions from NCp7 zinc fingers, inhibiting the maturation of virion proteins. Here, we show that the presence of DIBA-1 at the time of virus formation causes morphological malformations of the virus and reduces proviral DNA synthesis. Thus, it seems that DIBA-1 is responsible for a "core-freezing effect," as shown by electron microscopy analyses. DIBA-1 can also directly interfere with the fate of the newly made proviral DNA in a manner independent of its effects on virion core formation. These data strongly suggest that nueleocapsid protein is a prime target for new compounds aimed at inhibiting human immunodeficiency virus and other retroviruses.
引用
收藏
页码:10000 / 10009
页数:10
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