Determination of 18α-glycyrrhizin and 18β-glycyrrhizin in dog plasma by high-performance liquid chromatography

被引：20

作者：

Koga, K ^{[1
]}

Ohmachi, K

Kawashima, S

Takada, K

Murakami, M

机构：

[1] Hokuriku Univ, Fac Pharmaceut Sci, Dept Pharmaceut, Kanazawa, Ishikawa 9201181, Japan

[2] AMATO Pharmaceut Prod Ltd, R&D Dept, Fukuchiyama 6200932, Japan

[3] Kyoto Pharmaceut Univ, Dept Pharmacokinet, Kyoto 6078414, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 738卷 / 01期

关键词：

glycyrrhizin;

D O I：

10.1016/S0378-4347(99)00490-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method has been developed for the separation and determination of 18 alpha-glycyrrhizin (alpha-GZ) and 18 beta-glycyrrhizin (beta-GZ) in dog plasma. The two compounds were separated on a reversed-phase column and detected by UV absorption at 254 nm. The mobile phase was a mixture of water-methanol-60% perchloric acid (45:55:0.5, v/v) and was adjusted to pH 8.0 with 25% ammonia solution. Indomethacin was added to the plasma as an internal standard. Methanol was selected for the extraction of both the compounds and internal standard. alpha-GZ and beta-GZ could be precisely determined in concentration of 1 mg/ml in a 0.1 ml sample. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：165 / 168

页数：4