Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-γ and IL-10

被引：20

作者：

Brandao, Geane Peroni ^{[1
]}

Melo, Maria Norma ^{[1
]}

Gazzinelli, Ricardo Tostes ^{[2
]}

Caetano, Braulia Costa ^{[2
]}

Ferreira, Adriana Melo ^{[1
]}

Silva, Leticia Azevedo ^{[1
]}

Almeida Vitor, Ricardo Wagner ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2009年 / 104卷 / 02期

关键词：

experimental toxoplasmosis; reinfection; recombinant strains; cytokine; CHRONIC INFECTION; BRAZIL; ENCEPHALITIS; LYMPHOCYTES; PARASITE; MODEL;

D O I：

10.1590/S0074-02762009000200017

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

To assess reinfection of BALB/c mice with different Toxoplasma gondii strains, the animals were prime infected with the non-virulent D8 strain and challenged with virulent recombinant strains. Thirty days after challenge, brain cysts were obtained from surviving BALB/c mice and inoculated in Swiss mice to obtain tachyzoites for DNA extraction and PCR-RFLP analysis to distinguish the different T. gondii strains present in possible co-infections. Anti-Toxoplasma immune responses were evaluated in D8-primed BALB/c mice by detecting IFN-gamma and IL-10 produced by T cells and measuring immunoglobulin levels in serum samples. PCR-RFLP demonstrated that BALB/c mice were reinfected with the EGS strain at 45 days post prime infection (dpi) and with the EGS and CH3 strains at 180 dpi. High levels of IFN-gamma were detected after D8 infection, with no significant difference between 45 and 180-day intervals. However, higher IL-10 levels and higher plasmatic IgG1 and IgA were detected from samples obtained 180 days after infection. BALB/c mice were susceptible to reinfection with different recombinant T. gondii strains and this susceptibility correlated with enhancement of IL-10 production.

引用

页码：241 / 245

页数：5

共 23 条

[1] Chronic infection with Toxoplasma gondii does not prevent acute disease or colonization of the brain with tissue cysts following reinfection with different strains of the parasite [J].