Continuous spectrophotometric method for determining monophenolase and diphenolase activities of pear polyphenoloxidase

被引：53

作者：

Espin, JC ^{[1
]}

Morales, M ^{[1
]}

Varon, R ^{[1
]}

Tudela, J ^{[1
]}

GarciaCanovas, F ^{[1
]}

机构：

[1] UNIV CASTILLA LA MANCHA, ESCUELA POLITECN ALBACETE, E-13071 CIUDAD REAL, SPAIN

来源：

JOURNAL OF FOOD SCIENCE | 1996年 / 61卷 / 06期

关键词：

pear; polyphenoloxidase; monophenols; diphenols; MBTH;

D O I：

10.1111/j.1365-2621.1996.tb10955.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A continuous spectrophotometric method was based on the coupling reaction between 3-methyl-2-benzothiazolinone hydrazone (MBTH) and the quinone product of the oxidation of p-hydroxyphenyl propionic acid (PHPPA) or 3,4-dihydroxyphenyl propionic acid (DHPPA) in the presence of polyphenol oxidase. The monophenolase activity of pear PPO was characterized for the first time. Solubility and stability of the adduct formed at the optimum pH (4.3) enabled the system to reach steady-state, making it possible to determine monophenolase activity with short lag periods. This, together with the value of epsilon for the MBTH-quinone adduct, makes this method more sensitive than other continuous methods for assaying monophenolase and diphenolase activities.

引用

页码：1177 / +

页数：1

共 45 条

[1] [Anonymous], 1980, ANAL CHEM, V52, P2242
[2] BORDIER C, 1981, J BIOL CHEM, V256, P1604
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] MECHANISM OF THE OXIDATION OF NADH BY QUINONES - ENERGETICS OF ONE-ELECTRON AND HYDRIDE ROUTES
CARLSON, BW
MILLER, LL
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (02) : 479 - 485
[5] DUCKWORTH HW, 1970, J BIOL CHEM, V245, P1613
[6] A SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF THE CATECHOLASE ACTIVITY OF TYROSINASE AND SOME OF ITS APPLICATIONS
ELBAYOUMI, MA
FRIEDEN, E
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1957, 79 (18) : 4854 - 4858
[7] ENDRENYI L, 1981, KINETIC DATA ANAL DE
[8] RAPID ASSAY FOR CATECHOL OXIDASE AND LACCASE USING 2-NITRO-5-THIOBENZOIC ACID
ESTERBAUER, H
SCHWARZL, E
HAYN, M
[J]. ANALYTICAL BIOCHEMISTRY, 1977, 77 (02) : 486 - 494
[9] GARCIACARMONA F, 1979, ANAL BIOCHEM, V95, P433
[10] NEW SPECTROPHOTOMETRIC ASSAY FOR POLYPHENOL OXIDASE ACTIVITY
GAUILLARD, F
RICHARDFORGET, F
NICOLAS, J
[J]. ANALYTICAL BIOCHEMISTRY, 1993, 215 (01) : 59 - 65

← 1 2 3 4 5 →