LNA-Modified Primers Drastically Improve Hybridization to Target RNA and Reverse Transcription

被引：19

作者：

Fratczak, Agata ^{[1
]}

Kierzek, Ryszard ^{[1
]}

Kierzek, Elzbieta ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, PL-61704 Poznan, Poland

来源：

BIOCHEMISTRY | 2009年 / 48卷 / 03期

关键词：

LOCKED NUCLEIC-ACIDS; ESCHERICHIA-COLI; THERMODYNAMIC PARAMETERS; RNA/RNA HETERODUPLEXES; PCR PRIMERS; OLIGONUCLEOTIDES; GENE;

D O I：

10.1021/bi8021069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Knowledge about the structure of RNA is crucial to understanding its biological activities. Very often, the presence of unusually thermodynamically stable structural fragments in RNAs, such as hairpins, makes it impossible to apply primer extension to visualize the results of chemical mapping experiments. However, replacement of DNA primers with LNA-modified primers overcomes this limitation. This approach was tested successfully on regulatory OxyS RNA and DsrA RNA from Escherichia coli.

引用

页码：514 / 516

页数：3

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