Characterization of the major metalloprotease isolated from the venom of the northern Pacific rattlesnake, Crotalus viridis oreganus

Rattlesnake venoms typically contain several different metalloproteases, some of which are hemorrhagic toxins. Metalloproteases contribute significantly to the often severe necrotic changes in tissues following envenomation, and these prominent components are important to the predigestive role of venoms. Venom of the northern Pacific rattlesnake (Crotalus viridis oreganus) contains at least five distinct metalloproteases, and the dominant protease (trivial name, CVO protease V) has been isolated and characterized as being a single polypeptide chain acidic protein with a molecular mass of 61 kDa and a pH optimum of similar to 9.0. It catalyzes the hydrolysis of several protein substrates, including casein, and is inhibited by metal chelators such as EDTA, EGTA and 1,10-phenanthroline but not by serine protease inhibitors such as PMSF. Calcium is present at a molar ratio of approximately 1:1, but, unlike other described venom metalloproteases, this protease does not appear to contain zinc. Caseinolytic activity is not significantly inhibited by citrate (at pH 9.0) at levels up to 2.0 mM; at 100 mM citrate (at pH 9.0) more than 65% of activity is retained. It is partially inhibited by nanomolar concentrations of ATP, but higher amounts (micromolar) do not result in further inhibition of activity. The protease shows fibrinolytic and fibrinogenolytic activity, but is only weakly hemorrhagic in rats. When stored in solution for long periods it undergoes autolytic degradation. This protease or a homolog appears to be present in venoms from several rattlesnake species but is not present in venoms from juvenile C. v. oreganus. The presence of this component in venoms from adult Pacific rattlesnakes is responsible for the ape-related increase in metalloprotease activity of the crude venom.