Cloning and partial characterization of a Boophilus microplus (Acari: Ixodidae) glutathione S-transferase

被引:49
作者
de Lima, MFR
Ferreira, CAS
de Freitas, DRJ
Valenzuela, JG
Masuda, A
机构
[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande Sul, BR-91501970 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Dept Bioquim, BR-91501970 Porto Alegre, RS, Brazil
[3] NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA
[4] Univ Fed Rio Grande do Sul, Dept Mol Biol & Biotecnol, BR-91501970 Porto Alegre, RS, Brazil
关键词
Boophilus microplus; glutathione S-transferase; ectoparasites; cDNA cloning;
D O I
10.1016/S0965-1748(01)00157-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA of glutathione S-transferase (GST) was isolated from a cDNA library of salivary glands of Boophilus microplus. The recombinant protein was purified by glutathione affinity chromatography and assayed upon the chromogenic substrate CDNB. The 864 bp cloned fragment was sequenced and showed an open reading frame coding for a protein of 220 amino acids. Expression of the GST gene was tested by RT-PCR in tick tissues and larvae mRNA. Comparison of the deduced amino acid sequence with GSTs from other species revealed that the enzyme is closely related to the mammalian class mu GSTs. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:747 / 754
页数:8
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