Use of green fluorescent protein for detection of transformed shoots and homozygous offspring

被引：40

作者：

Molinier, J

Himber, C

Hahne, G

机构：

[1] CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France

[2] Univ Strasbourg 1, F-67084 Strasbourg, France

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 03期

关键词：

green fluorescent protein; marker gene; Nicotiana tabacum; plant transformation; reporter gene;

D O I：

10.1007/s002990050002

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds and seedlings of the T1 generation. These results were confirmed by genetic analysis. Optimized conditions for GFP analysis of stable transformants are presented.

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页码：219 / 223

页数：5

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