beta-galactosidase transferase activity in ice and use of vinyl-beta-D-galactoside as donor

被引：16

作者：

ChiffoleauGiraud, V ^{[1
]}

Spangenberg, P ^{[1
]}

Rabiller, C ^{[1
]}

机构：

[1] FAC SCI & TECH,UNITE RECH BIOCATALYSE,UPRES 2161,F-44322 NANTES 3,FRANCE

来源：

TETRAHEDRON-ASYMMETRY | 1997年 / 8卷 / 12期

关键词：

D O I：

10.1016/S0957-4166(97)00194-8

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The ability of vinyl-beta-D-galactoside as a donor in transglycosylation reactions catalysed by the beta-glycosidase from Aspergillus oryzae using allylic alcohol and methyl-alpha-galactoside as accepters is tested. A kinetic study made in comparison with another donor, the 2-nitrophenyl-beta-D-galactoside, shows that the use of the latter leads at room temperature to better yields than those obtained from the former. A reverse situation is observed in ice at -7 degrees C, conditions in which the yield for transglycosylation can be enhanced from 59% to 82% with methyl-alpha-galactoside as an acceptor. (C) 1997 Elsevier Science Ltd.

引用

页码：2017 / 2023

页数：7

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