Fibrin is a regulator of Schwann cell migration after sciatic nerve injury in mice

被引：49

作者：

Akassoglou, K

Akpinar, P

Murray, S

Strickland, S

机构：

[1] NYU, Med Ctr, Mol Neurobiol Program, Skirball Inst Biomol Med, New York, NY 10016 USA

[2] Rockefeller Univ, Lab Neurobiol & Genet, New York, NY 10021 USA

来源：

NEUROSCIENCE LETTERS | 2003年 / 338卷 / 03期

基金：

美国国家卫生研究院;

关键词：

sciatic nerve injury; extracellular matrix; plasminogen; biomaterials; arg-gly-asp peptide; regeneration;

D O I：

10.1016/S0304-3940(02)01387-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Fibrin, derived from the blood protein fibrinogen, is deposited in the sciatic nerve after injury and retards functional regeneration. Since Schwann cell migration is critical for remyelination of injured nerves, we investigated the effects of fibrin in this process. In vivo experiments showed that fibrin co-localizes with fibronectin deposition in the injured sciatic nerve. In vitro migration assays demonstrated that fibrin alone is not a permissive substrate for Schwann cell migration. Furthermore, migration assays of Schwann cells on mixed fibrin/fibronectin substrates showed that fibrin has a dose dependent inhibitory effect on Schwann cell migration on fibronectin. Our results show that fibrin, deposited in the sciatic nerve after injury, changes the composition of the extracellular matrix and inhibits Schwann cell migration. This negative effect of fibrin should be considered in the therapeutic application of biomaterials based on fibrin matrices. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：185 / 188

页数：4

共 13 条

[1]

Ahmed Z, 2000, CELL MOTIL CYTOSKEL, V46, P6

[2] Tissue plasminogen activator-mediated fibrinolysis protects against axonal degeneration and demyelination after sciatic nerve injury [J].