Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats

Objective. To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Design. Randomized, controlled study. Setting. Animal care facility procedure room. Subjects. Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g. Interventions. Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lavage fluid. Measurements and Main Results., Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p < .05 similar to p < .001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5'-triphosphatase inhibitor) was added before FDP pretreatment. Conclusions., Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.