A HaemAtlas: characterizing gene expression in differentiated human blood cells

被引：189

作者：

Watkins, Nicholas A. ^{[1
]}

Gusnanto, Arief ^{[2
]}

de Bono, Bernard ^{[3
]}

De, Subhajyoti ^{[4
]}

Miranda-Saavedra, Diego ^{[5
]}

Hardie, Debbie L. ^{[6
]}

Angenent, Will G. J. ^{[7
]}

Attwood, Antony P. ^{[7
]}

Ellis, Peter D. ^{[7
]}

Erber, Wendy ^{[8
]}

Foad, Nicola S. ^{[1
]}

Garner, Stephen F. ^{[1
]}

Isacke, Clare M. ^{[9
]}

Jolley, Jennifer ^{[1
]}

Koch, Kerstin ^{[1
]}

Macaulay, Iain C. ^{[1
]}

Morley, Sarah L. ^{[1
]}

Rendon, Augusto ^{[1
]}

Rice, Kate M. ^{[7
]}

Taylor, Niall ^{[1
]}

Thijssen-Timmer, Daphne C. ^{[10
]}

Tijssen, Marloes R. ^{[10
]}

van der Schoot, C. Ellen ^{[10
]}

Wernisch, Lorenz ^{[2
]}

Winzer, Thilo ^{[1
]}

Dudbridge, Frank ^{[2
]}

Buckley, Christopher D. ^{[6
]}

Langford, Cordelia F. ^{[7
]}

Teichmann, Sarah ^{[4
]}

Goettgens, Berthold ^{[5
]}

Ouwehand, Willem H. ^{[1
,7
]}

机构：

[1] Univ Cambridge, Dept Haematol, Natl Hlth Serv Blood & Transplant, Cambridge CB2 2PT, England

[2] Univ Forvie Site, MRC, Biostat Unit, Cambridge, England

[3] European Bioinformat Inst, Cambridge, England

[4] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[5] Wellcome Trust Res Labs, Cambridge, England

[6] Univ Birmingham, Div Immun & Infect, MRC, Ctr Immune Regulat, Birmingham, W Midlands, England

[7] Wellcome Trust Sanger Inst, Cambridge, England

[8] Cambridge Univ Hosp, Natl Hlth Serv Fdn Trust, Addenbrookes Hosp, Dept Haematol, Cambridge, England

[9] Inst Canc Res, Breakthrough Breast Canc Res Ctr, London, England

[10] Univ Amsterdam, Acad Med Ctr, Dept Expt Immunohaematol, Sanquin Res & Landsteiner Lab, NL-1105 AZ Amsterdam, Netherlands

来源：

BLOOD | 2009年 / 113卷 / 19期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

IN-VIVO; RECEPTOR; PROTEIN; MIGRATION; FAMILY; TRANSCRIPTION; STIMULATION; ENDOTHELIUM; PREDICTIONS; NEUTROPHILS;

D O I：

10.1182/blood-2008-06-162958

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Hematopoiesis is a carefully controlled process that is regulated by complex networks of transcription factors that are, in part, controlled by signals resulting from ligand binding to cell-surface receptors. To further understand hematopoiesis, we have compared gene expression profiles of human erythroblasts, megakaryocytes, B cells, cytotoxic and helper T cells, natural killer cells, granulocytes, and monocytes using whole genome microarrays. Abioinformatics analysis of these data was performed focusing ontranscription factors, immunoglobulin superfamily members, and lineage-specific transcripts. We observed that the numbers of lineage-specific genes varies by 2 orders of magnitude, ranging from 5 for cytotoxic T cells to 878 for granulocytes. In addition, we have identified novel coexpression patterns for key transcription factors involved in hematopoiesis (eg, GATA3-GFI1 and GATA2-KLF1). This study represents the most comprehensive analysis of gene expression in hematopoietic cells to date and has identified genes that play key roles in lineage commitment and cell function. The data, which are freely accessible, will be invaluable for future studies on hematopoiesis and the role of specific genes and will also aid the understanding of the recent genome-wide association studies. (Blood. 2009;113:e-e9)

引用

页码：E1 / E9

页数：9

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