A new molecular network comprising PU.1, interferon regulatory factor proteins and miR-342 stimulates ATRA-mediated granulocytic differentiation of acute promyelocytic leukemia cells

被引：70

作者：

De Marchis, M. L. ^{[1
]}

Ballarino, M. ^{[1
]}

Salvatori, B. ^{[1
]}

Puzzolo, M. C. ^{[1
]}

Bozzoni, I. ^{[1
,2
]}

Fatica, A. ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Genet & Mol Biol, Inst Pasteur Cenci Bolognetti, I-00185 Rome, Italy

[2] CNR, Inst Mol Biol & Pathol, Rome, Italy

来源：

LEUKEMIA | 2009年 / 23卷 / 05期

关键词：

microRNA; APL; ATRA; PU.1; IRF-1; IRF-9; GENE-EXPRESSION; TRANSCRIPTION FACTORS; MICRORNAS; PROMOTER; BINDING; FAMILY; CANCER; ALPHA;

D O I：

10.1038/leu.2008.372

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In the acute promyelocytic leukemia (APL) bearing the t(15; 17), all-trans-retinoic acid (ATRA) treatment induces granulocytic maturation and complete remission of leukemia. We identified miR-342 as one of the microRNAs (miRNAs) upregulated by ATRA during APL differentiation. This miRNA emerged as a direct transcriptional target of the critical hematopoietic transcription factors PU.1 and interferon regulatory factor (IRF)-1 and IRF-9. IRF-1 maintains miR-342 at low levels, whereas the binding of PU.1 and IRF-9 in the promoter region following retinoic ATRA-mediated differentiation, upregulates miR-342 expression. Moreover, we showed that enforced expression of miR-342 in APL cells stimulated ATRA-induced differentiation. These data identified miR-342 as a new player in the granulocytic differentiation program activated by ATRA in APL. Leukemia (2009) 23, 856-862; doi:10.1038/leu.2008.372; published online 8 January 2009

引用

页码：856 / 862

页数：7

共 22 条

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