Interactions between FGF and Wnt signals and Tbx3 gene expression in mammary gland initiation in mouse embryos

被引:91
作者
Eblaghie, MC
Song, SJ
Kim, JY
Akita, K
Tickle, C
Jung, HS [1 ]
机构
[1] Yonsei Univ, Yonsei Ctr Biotechnol,Div Anat & Dev Biol, Korea 21 Project Med Sci,Oral Sci Res Ctr, Dept Oral Biol,Coll Dent,Res Ctr Orofacial Hard T, Seoul 120749, South Korea
[2] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
[3] Tokyo Med & Dent Univ, Grad Sch, Unit Biostruct Sci, Tokyo, Japan
[4] Univ Dundee, Fac Life Sci, Div Cell & Dev Biol, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland
关键词
embryonic mammary gland; Fgfs; T-box; Wnt;
D O I
10.1111/j.0021-8782.2004.00309.x
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Interactions between Wnts, Fgfs and Tbx genes are involved in limb initiation and the same gene families have been implicated in mammary gland development. Here we explore how these genes act together in mammary gland initiation. We compared expression of Tbx3, the gene associated with the human condition ulnar-mammary syndrome, expression of the gene encoding the dual-specificity MAPK phosphatase Pyst1/MKP3, which is an early response to FGFR1 signalling (as judged by sensitivity to the SU5402 inhibitor), and expression of Lef1, encoding a transcription factor mediating Wnt signalling and the earliest gene so far known to be expressed in mammary gland development. We found that Tbx3 is expressed earlier than Lef1 and that Pyst1 is also expressed early but only transiently. Patterns of expression of Tbx3, Pyst1 and Lef1 in different glands suggest that the order of mammary gland initiation is 3, 4, 1, 2 and 5. Consistent with expression of Pyst1 in the mammary gland, we detected expression of Fgfr1b, Fgf8 and Fgf9 in both surface ectoderm and mammary bud epithelium, and Fgf4 and Fgf17 in mammary bud epithelium. Beads soaked in FGF-8 applied to the flank of mouse embryos, at a stage just prior to mammary bud initiation, induce expression of Pyst1 and Lef1 and maintain Tbx3 expression in flank tissue surrounding the bead. Grafting beads soaked in the FGFR1 inhibitor, SU5402, abolishes Tbx3, Pyst1 and Lef1 expression, supporting the idea that FGFR1 signalling is required for early mammary gland initiation. We also showed that blocking Wnt signalling abolishes Tbx3 expression but not Pyst1 expression. These data, taken together with previous findings, suggest a model in which Tbx3 expression is induced and maintained in early gland initiation by both Wnt and Fgf signalling through FGFR1.
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页码:1 / 13
页数:13
相关论文
共 50 条
[1]   Mutations in human TBX3 alter limb, apocrine and genital development in ulnar-mammary syndrome [J].
Bamshad, M ;
Lin, RC ;
Law, DJ ;
Watkins, WS ;
Krakowiak, PA ;
Moore, ME ;
Franceschini, P ;
Lala, R ;
Holmes, LB ;
Gebuhr, TC ;
Bruneau, BG ;
Schinzel, A ;
Seidman, JG ;
Seidman, CE ;
Jorde, LB .
NATURE GENETICS, 1997, 16 (03) :311-315
[2]   Induction of mammary gland development in estrogen receptor-α knockout mice [J].
Bocchinfuso, WP ;
Lindzey, JK ;
Hewitt, SC ;
Clark, JA ;
Myers, PH ;
Cooper, R ;
Korach, KS .
ENDOCRINOLOGY, 2000, 141 (08) :2982-2994
[3]   WNT-4 EXPRESSION INDUCES A PREGNANCY-LIKE GROWTH-PATTERN IN RECONSTITUTED MAMMARY-GLANDS IN VIRGIN MICE [J].
BRADBURY, JM ;
EDWARDS, PAW ;
NIEMEYER, CC ;
DALE, TC .
DEVELOPMENTAL BIOLOGY, 1995, 170 (02) :553-563
[4]  
Casey ES, 1998, DEVELOPMENT, V125, P3887
[5]   T-box gene products are required for mesenchymal induction of epithelial branching in the embryonic mouse lung [J].
Cebra-Thomas, JA ;
Bromer, J ;
Gardner, R ;
Lam, GK ;
Sheipe, H ;
Gilbert, SF .
DEVELOPMENTAL DYNAMICS, 2003, 226 (01) :82-90
[6]  
Chapman DL, 1996, DEV DYNAM, V206, P379, DOI 10.1002/(SICI)1097-0177(199608)206:4<379::AID-AJA4>3.0.CO
[7]  
2-F
[8]   MURINE WNT-11 AND WNT-12 HAVE TEMPORALLY AND SPATIALLY RESTRICTED EXPRESSION PATTERNS DURING EMBRYONIC-DEVELOPMENT [J].
CHRISTIANSEN, JH ;
DENNIS, CL ;
WICKING, CA ;
MONKLEY, SJ ;
WILKINSON, DG ;
WAINWRIGHT, BJ .
MECHANISMS OF DEVELOPMENT, 1995, 51 (2-3) :341-350
[9]   FIBROBLAST GROWTH-FACTORS INDUCE ADDITIONAL LIMB DEVELOPMENT FROM THE FLANK OF CHICK-EMBRYOS [J].
COHN, MJ ;
IZPISUABELMONTE, JC ;
ABUD, H ;
HEATH, JK ;
TICKLE, C .
CELL, 1995, 80 (05) :739-746
[10]   Coordinate expression of Fgf8, Otx2, Bmp4, and Shh in the rostral prosencephalon during development of the telencephalic and optic vesicles [J].
Crossley, PH ;
Martinez, S ;
Ohkubo, Y ;
Rubenstein, JLR .
NEUROSCIENCE, 2001, 108 (02) :183-206