3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy

被引：36

作者：

Amzallag, Arnaud ^{[1
]}

Vaillant, Cedric

Jacob, Mathews

Unser, Michael

Bednar, Jan

Kahn, Jason D.

Dubochet, Jacques

Stasiak, Andrzej

Maddocks, John H.

机构：

[1] Ecole Polytech Fed Lausanne, FSB IMB, Lab Computat & Visualizat Math & Mech, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Biomed Imaging Grp, LIB, CH-1015 Lausanne, Switzerland

[3] CNRS, UMR 5588, Spectrometrie Phys Lab, F-38402 St Martin Dheres, France

[4] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

[5] Univ Lausanne, Lab Ultrastruct Anal, CH-1015 Lausanne, Switzerland

来源：

NUCLEIC ACIDS RESEARCH | 2006年 / 34卷 / 18期

关键词：

D O I：

10.1093/nar/gkl675

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.

引用

页数：8

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