GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli

被引：26

作者：

Araújo, LA ^{[1
]}

Monteiro, RA ^{[1
]}

Souza, EA ^{[1
]}

Steffens, ABR ^{[1
]}

Rigo, LU ^{[1
]}

Pedrosa, FO ^{[1
]}

Chubatsu, LS ^{[1
]}

机构：

[1] Univ Fed Parana, Dept Biochem & Mol Biol, BR-81531990 Curitiba, Parana, Brazil

来源：

RESEARCH IN MICROBIOLOGY | 2004年 / 155卷 / 06期

关键词：

Azospirillum brasilense; NifA; PII-like protein; GlnK; GInZ; GlnB; nitrogen fixation;

D O I：

10.1016/j.resmic.2004.03.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Azospirillum brasilense transcription regulator NifA and the nitrogen-status signaling proteins GlnB, GlnZ and GlnK were expressed in Escherichia coli and analyzed for their ability to activate nif gene expression. When expressed separately, none of the proteins were able to activate nifH promoter expression in any tested conditions; in contrast, nifH expression was observed in cells grown in the absence of ammonium and oxygen and when expressing simultaneously NifA and GlnB proteins, but not when expressing NifA and GlnZ or GlnK. Our results show that the GlnB protein is required for transcription activation by Azospirillum brasilense NifA and it cannot be replaced by GlnZ or GlnK. (C) 2004 Elsevier SAS. All rights reserved.

引用

页码：491 / 495

页数：5

共 40 条

[1] Studies on the roles of GlnK and GlnB in regulating Klebsiella pneumoniae NifL-dependent nitrogen control
Arcondéguy, T
van Heeswijk, WC
Merrick, M
[J]. FEMS MICROBIOLOGY LETTERS, 1999, 180 (02) : 263 - 270
[2] Control of Azospirillum brasilense NifA activity by PII:: effect of replacing Tyr residues of the NifA N-terminal domain on NifA activity
Arsène, F
Kaminski, PA
Elmerich, C
[J]. FEMS MICROBIOLOGY LETTERS, 1999, 179 (02) : 339 - 343
[3] Modulation of NifA activity by P-II in Azospirillum brasilense: Evidence for a regulatory role of the NifA N-terminal domain
Arsene, F
Kaminski, PA
Elmerich, C
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (16) : 4830 - 4838
[4] CHARACTERIZATION OF THE KLEBSIELLA-PNEUMONIAE NITROGEN-FIXATION REGULATORY PROTEINS NIFA AND NIFL INVITRO
AUSTIN, S
HENDERSON, N
DIXON, R
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 187 (02): : 353 - 360
[5] Evidence for two possible glnB-type genes in Herbaspirillum seropedicae
Benelli, EM
Souza, EM
Funayama, S
Rigo, LU
Pedrosa, FO
[J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (14) : 4623 - 4626
[6] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7] BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5
[8] 16S Ribosomal DNA Characterization of Nitrogen-Fixing Bacteria Isolated from Banana (Musa spp.) and Pineapple (Ananas comosus (L.) Merril)
[J]. 2001, American Society for Microbiology (67)
[9] Structural homologues PII and Pz of Azospirillum brasilense provide intracellular signalling for selective regulation of various nitrogen-dependent functions
de Zamaroczy, M
[J]. MOLECULAR MICROBIOLOGY, 1998, 29 (02) : 449 - 463
[10] Coexistence of two structurally similar but functionally different P-II proteins in Azospirillum brasilense
deZamaroczy, M
Paquelin, A
Peltre, G
Forchhammer, K
Elmerich, C
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (14) : 4143 - 4149

← 1 2 3 4 →