Leaf senescence in Brassica napus: Cloning of senescence related genes by subtractive hybridisation

被引：214

作者：

BuchananWollaston, V

Ainsworth, C

机构：

[1] Department of Biological Sciences, Wye College, University of London, Wye, Ashford, Kent

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 33卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

leaf senescence; genes; gene expression; subtractive hybridisation; Brassica napus;

D O I：

10.1023/A:1005774212410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A subtractive hybridisation technique was developed to clone cDNAs representing genes that showed enhanced expression during leaf senescence in Brassica napus. A number of different genes were identified that, when analysed by northern hybridisation, showed different patterns of expression during leaf development but were all expressed at increased levels during senescence. Sequence analysis of these cDNAs showed that several types of genes were found including two different proteases, glutamine synthetase, ATP sulphurylase, catalase, metallothionein, ferritin and an antifungal protein. The possible roles of these gene products in the senescence process are discussed.

引用

页码：821 / 834

页数：14

共 54 条

[1] EXPRESSION, ORGANIZATION AND STRUCTURE OF THE GENES ENCODING THE WAXY PROTEIN (GRANULE-BOUND STARCH SYNTHASE) IN WHEAT [J].