A hyper-thermostable, alkaline lipase from Pseudomonas sp with the property of thermal activation

被引：52

作者：

Rathi, P ^{[1
]}

Bradoo, S ^{[1
]}

Saxena, RK ^{[1
]}

Gupta, R ^{[1
]}

机构：

[1] Univ Delhi, Dept Microbiol, New Delhi 110021, India

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 06期

关键词：

hyper-thermostability; lipase; mustard oil; Pseudomonas sp; thermal activation;

D O I：

10.1023/A:1005604617440

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A hyper-thermostable, alkaline lipase from a newly-isolated, mesophilic Pseudomonas sp. was optimal at pH 11 and at 90 degrees C. It had a half-life of more than 13 h at 90 degrees C. It was activated by 30% when heated at 90 degrees C for 2 h. The enzyme had a greater affinity for mustard oil (K-m = 40 mg ml(-1)) than for olive oil (K-m = 140 mg ml(-1)).

引用

页码：495 / 498

页数：4

共 12 条

[1]

Ghosh P. K., 1996, Science Progress, V79, P119

[2] PSEUDOMONAS LIPASES - BIOCHEMICAL-PROPERTIES AND MOLECULAR-CLONING [J].