Sequencing the maize genome

被引:46
作者
Martienssen, RA [1 ]
Rabinowicz, PD [1 ]
O'Shaughnessy, A [1 ]
McCombie, WR [1 ]
机构
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
基金
美国国家科学基金会;
关键词
D O I
10.1016/j.pbi.2004.01.010
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(o)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.
引用
收藏
页码:102 / 107
页数:6
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