Mechanical characterization of skeletal muscle myofibrils

A new instrument, based on a technique described previously, is presented for Studying mechanics of micron-scale preparations oi two to three myofibrils or single myofibrils from muscle, Forces-in the nanonewton to micronewton range are measurable with 0.5-ms time resolution. Programmed quick (200-mu s) steps or ramp length changes are applied to contracting myofibrils to-test mechanical properties. Individual striations can be monitored during force production and shortening. The active isometric force, force-velocity relationship, and force transients after rapid length steps were obtained from bundles of two to three myofibrils from rabbit psoas muscle. Contrary to some earlier reports on myofibrillar mechanics, these properties generally similar to expectations from studies on intact and skinned muscle fibers. Our experiments provide strong evidence that the mechanical properties of a fiber result from a simple summation of the myofibrillar force and shortening of independently contracting sarcomeres.