Multiplex Ligation-Dependent Probe Amplification of Uveal Melanoma: Correlation with Metastatic Death

PURPOSE. To evaluate multiplex ligation-dependent probe amplification (MLPA) of uveal melanoma as a predictive tool for metastatic death. METHODS. Uveal melanoma specimens of 73 patients treated between 1998 and 2000 were included. DNA samples were analyzed with MLPA evaluating 31 loci on chromosomes 1, 3, 6 and 8, and the results were correlated with metastatic death. RESULTS. The patients (27 women; 46 men) had a median age of 60.6 years and a median follow-up of 6.2 years. Metastatic death occurred in 28 patients, correlating most strongly with chromosome 3 losses and gains on 8q (Cox univariate analysis, P < 0.001). Chromosome 6, region p25, gains correlated with good survival (Cox univariate analysis, P = 0.003). Prediction of metastatic death was improved by considering equivocal chromosome 3 losses as abnormal and by taking account of multiple risk factors, such as 8q gains, tumor diameter, and histologic features indicative of high-grade malignancy. CONCLUSIONS. MLPA analysis of uveal melanoma predicts metastatic death if statistically insignificant losses of chromosome 3 are considered together with gains in 8q as well as clinical stage and histologic grade of malignancy. (Invest Ophthalmol Vis Sci. 2009;50:3048-3055) DOI: 10.1167/iovs.08-3165