Formation of 2′-deoxyuridine hydrates upon exposure of nucleosides to gamma radiation and UVC-irradiation of isolated and cellular DNA

被引：20

作者：

Douki, T ^{[1
]}

Vadesne-Bauer, G ^{[1
]}

Cadet, J ^{[1
]}

机构：

[1] CEA, Dept Rech Fondamentale Matiere Condensee, DSM, UMR 5046,Serv Chim Inorgan & Biol,Lab Les Acides, F-38054 Grenoble 9, France

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2002年 / 1卷 / 08期

关键词：

D O I：

10.1039/b201612e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The two diastereoisomers of 6-hydroxy-5,6-dihydro-2'-deoxyuridine (dUrd hydrates) are produced upon deamination of the related 2'-deoxycytidine derivatives. Liquid chromatography coupled to tandem mass spectrometry was used to quantify dUrd hydrates. Their rate constant of formation within UVC-irradiated solutions of dCyd was first determined. Their formation was also shown to occur within solutions of either dUrd or dCyd exposed to gamma-rays in the absence of oxygen. dUrd hydrates were then quantified within isolated and cellular DNA exposed to UVC light. In both cases, their yield of formation was found to be, at least, 2 orders of magnitude lower than the overall yield of bipyrimidine photoproducts such as cyclobutane dimers and (6-4) photoproducts.

引用

页码：565 / 569

页数：5

共 29 条

[1]

[Anonymous], PHOTOPHYSIOLOGY

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