Phenotypic Characterization and Invasive Properties of Synovial Fluid-Derived Adherent Cells in Rheumatoid Arthritis

被引：14

作者：

Ahn, Joong Kyong ^{[1
]}

Kim, HyungJin ^{[1
]}

Lee, JaeJoon ^{[1
]}

Bae, Eun-Kyung ^{[2
]}

Cha, Hoon-Suk ^{[1
]}

Koh, Eun-Mi ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Med, Seoul 135710, South Korea

[2] Sungkyunkwan Univ, Sch Med, Ctr Mol Med, Samsung Biomed Res Inst, Seoul 135710, South Korea

来源：

INFLAMMATION | 2008年 / 31卷 / 06期

关键词：

rheumatoid arthritis; synovial fluid; synovial tissue; fibroblast-like synoviocytes;

D O I：

10.1007/s10753-008-9087-x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

The present study aimed at characterizing the phenotype and functions of adherent synovial fluid (SF) cells derived from rheumatoid arthritis (RA), comparing with fibroblast-like synoviocytes (FLS) derived from RA synovial tissue (ST). Adherent SF-derived cells were spindle-shaped from passages 1-6 under light microscopy. The cell surface marker pro. le in SF-derived cells from passage 1-6 was similar to that of ST-derived FLS. Levels of MMP-1 and MMP-3 were not significantly different between SF-derived cells and ST-derived FLS (p=0.20 and p=0.40, respectively). There was no significant difference in the optical density value between two cell types in the cell invasion assay (p=0.10). SF-derived adherent cells have a. broblast-like phenotype from very early culture passages and have the potential to produce MMPs with the invasive capacity to degrade cartilage, identical to ST-derived FLS. Therefore, these cells could substitute for ST-derived FLS in studying the pathogenesis of RA.

引用

页码：365 / 371

页数：7

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