Miscibility of DPPC and DPPA in monolayers at the air/water interface

被引:27
作者
Estrela-Lopis, I
Brezesinski, G
Möhwald, H
机构
[1] Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany
[2] Univ Leipzig, Inst Med Phys & Biophys, D-04103 Leipzig, Germany
关键词
phospholipids; monolayers; X-ray diffraction; miscibility; phase diagram;
D O I
10.1016/j.chemphyslip.2004.04.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Monolayers of mixtures of 1,2-dipalmitoylphosphatidylcholine (DPPC) as the substrate and 1,2-dipalmitoylphosphatidic acid (DPPA) as the product of the hydrolysis reaction catalyzed by phospholipase D (PLD) were investigated in the presence of Ca2+. The miscibility behavior and the microstructure of mixed domains have been studied by gracing incidence X-ray diffraction (GIXD), Brewster angle microscopy and film balance measurements. The phase diagram reveals partial miscibility on both sides and a wide miscibility gap, which becomes narrower at high pressure. At low pressure, the segregation of condensed DPPA-rich domains in a fluid-like DPPC matrix was detected already at small DPPA concentrations and their structure was determined. A small amount of DPPC mixed into the segregated DPPA domains induces the transformation from rectangular to an oblique unit cell and increases the tilt angle in the condensed domains. At high pressure, two types of condensed phase domains were found: DPPC-rich and DPPA-rich. A drastic reduction of the tilt angle in the DPPC-rich domains with increasing amount of DPPA was observed. The decrease of the tilt angle must be connected with a change of the head group conformation of DPPC in such mixed domains. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:71 / 80
页数:10
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