Nickel-Ion-Mediated Control of the Stoichiometry of His-Tagged Protein/Nanoparticle Interactions

被引：29

作者：

De, Mrinmoy ^{[1
]}

Rana, Subinoy ^{[1
]}

Rotello, Vincent M. ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2009年 / 9卷 / 02期

基金：

美国国家科学基金会;

关键词：

fluorescence; nanocomposites; nanoparticles; proteins; selectivity; MODIFIED MAGNETIC NANOPARTICLES; GREEN FLUORESCENT PROTEIN; GOLD NANOPARTICLES; QUANTUM DOTS; FUNCTIONALIZATION; IDENTIFICATION; PURIFICATION; BINDING; CLUSTER;

D O I：

10.1002/mabi.200800289

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction between synthetic materials and biomolecules plays an important role in biomedical and pathological sciences. An important issue in these interactions is control of stoichiometry. The interaction between NTA ligands and proteins with six consecutive His residues has been widely used for protein purification. Control of stoichiometry is an important issue in applying this recognition strategy to the creation of defined nanoparticle-protein conjugates. In this communication we report the direct control of particle-protein stoichiometry through variation of nickel chloride concentration, as demonstrated through fluorscence and gel electrophoresis.

引用

收藏

页码：174 / 178

页数：5

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