Very high pressure HPLC with 1 mm id columns

被引：58

作者：

Colón, LA

Cintrón, JM

Anspach, JA

Fermier, AM

Swinney, KA

机构：

[1] SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA

[2] Johnson & Johnson Pharmaceut Res & Dev, Sci & New Technol Analyt Dev, Raritan, NJ 08869 USA

来源：

ANALYST | 2004年 / 129卷 / 06期

关键词：

D O I：

10.1039/b405242k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Theoretical calculations and experimental data indicate that very high pressure HPLC can be performed using 1 and 1.5 mm id columns, and contrary to previous beliefs, the frictional heating generated does not appear to be detrimental to the separation.

引用

页码：503 / 504

页数：2

共 17 条

[1] Packing and stationary phase design for capillary electroendosmotic chromatography (CEC) [J].

Adam, T ;

Lüdtke, S ;

Unger, KK .

CHROMATOGRAPHIA, 1999, 49 (Suppl 1) :S49-S55

[2] Organo-silica nano-particles used in ultrahigh-pressure liquid chromatography [J].

Cintrón, JM ;

Colón, LA .

ANALYST, 2002, 127 (06) :701-704

[3] Effect of pressure-induced ionization, partitioning, and complexation on solute retention in reversed-phase liquid chromatography [J].

Evans, CE ;

Davis, JA .

ANALYTICA CHIMICA ACTA, 1999, 397 (1-3) :163-172

[4]

GIDDINGS JC, 1991, UNIFIED SEPARATION S, pCH4

[5] ULTIMATE LIMITS IN HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

HALASZ, I ;

ENDELE, R ;

ASSHAUER, J .

JOURNAL OF CHROMATOGRAPHY, 1975, 112 (OCT29) :37-60

[6] Fast analytical-scale separations by capillary electrophoresis and liquid chromatography [J].

Kennedy, RT ;

German, I ;

Thompson, JE ;

Witowski, SR .

CHEMICAL REVIEWS, 1999, 99 (10) :3081-+

[7] Ultrafast reversed-phase high-performance liquid chromatographic separations: An overview [J].

Kirkland, JJ .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2000, 38 (12) :535-544

[8]

Lippert JA, 1999, J MICROCOLUMN SEP, V11, P631, DOI 10.1002/(SICI)1520-667X(199911)11:9<631::AID-MCS1>3.3.CO

[9]

2-9

[10] Application of 0.5-mu m porous silanized silica beads in electrochromatography [J].

Ludtke, S ;

Adam, T ;

Unger, KK .

JOURNAL OF CHROMATOGRAPHY A, 1997, 786 (02) :229-235

← 1 2 →