Functional LCAT is not required for macrophage cholesterol efflux to human serum

Objectives: To evaluate the capacity of serum from carriers of LCAT gene Mutations to promote cell cholesterol efflux through the ABCA1, ABCG1, and SR-B1 pathways. Methods: Serum was obtained from 41 carriers of mutant LCAT alleles (14 carriers of two mutant LCAT alleles and 27 heterozygotes) and 10 non-carrier relatives (controls). The capacity of serum to promote cholesterol efflux was tested in pathway-specific cell models. Results: LCAT deficient sera were significantly More efficient than control sera in promoting cell cholesterol efflux via ABCA1 (3.1 +/- 0.3% for carriers Of two mutant LCAT alleles and 2.6 +/- 0.2% for heterozygotes vs. 1.5 +/- 0.4% for controls), and less efficient in promoting ABCG1 - and SR-B1-mediated cholesterol efflux. The enhanced capacity of LCAT deficient serum for ABCA1 efflux is explained by the increased content of pre beta-HDL, as indicated by the significant positive correlation between ABCA1 efflux and serum pre beta-HDL content (R=0.468, P<0.001). Moreover, chymase treatment of LCAT deficient serum selectively degraded pre beta-HDL and completely abolished ABCA1 efflux. Despite the remarkable reductions in serum HDL levels, LCAT deficient sera were as effective as control sera in removing mass cholesterol from cholesterol-loaded macrophages. Conclusions: Serum from carriers of LCAT gene mutations has the same capacity of control serum to decrease the cholesterol content of cholesterol-loaded macrophages due to a greater cholesterol efflux capacity via ABCA1. (C) 2008 Elsevier Ireland Ltd. All rights reserved.