Effect of γ-hydroxypropano deoxyguanosine, the major acrolein-derived adduct, on monomolecular quadruplex structure of telomeric repeat d(TTAGGG)4

被引：4

作者：

D'Isa, G

Galeone, A

Oliviero, G

Piccialli, G

Varra, M

Mayol, L

机构：

[1] Univ Naples Federico II, Dipartimento Chim Sostanze Nat, Fac Farm, I-80131 Naples, Italy

[2] Univ Naples Federico II, Dipartimento Chim Sostanze Nat, Fac Sci Biotecnol, I-80131 Naples, Italy

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 21期

关键词：

acrolein; telomeric repeat; quadruplex;

D O I：

10.1016/j.bmcl.2004.07.074

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The three oligodeoxyribonucleotides (ODNs) a-c, having the telomeric repeat d(TTAGGG)(4) sequence and incorporating gamma-hydroxypropano deoxyguanosine at different positions, were synthesized. Gel electrophoresis and CD analyses indicated that the ODNs assume monomolecular quadruplex structures in Na+ and in K+ buffers. The T-m values, obtained by CD melting experiments, showed that the presence of the acrolein-dG adduct into the ODN b decreases the thermal stability of the monomolecular quadruplex structure in Na+ solution, whereas for a and c no significant effect could be detected in the same experimental conditions. On the contrary, all ODNs a-d show the same behaviour in K+ buffer. These findings are briefly discussed. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：5417 / 5421

页数：5

共 49 条

[1] Human neutrophils employ the myeloperoxidase-hydrogen peroxide-chloride system to convert hydroxy-amino acids into glycolaldehyde, 2-hydroxypropanal, and acrolein - A mechanism for the generation of highly reactive alpha-hydroxy and alpha,beta-unsaturated aldehydes by phagocytes at sites of inflammation [J].