Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton

被引:323
作者
Lillemeier, Bjorn F.
Pfeiffer, Janet R.
Surviladze, Zurab
Wilson, Bridget S.
Davis, Mark M.
机构
[1] Stanford Univ, Sch Med, Howard Hughes Med Inst, Beckman Ctr, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA
[3] Univ New Mexico, Dept Pathol, Albuquerque, NM 87131 USA
[4] Univ New Mexico, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA
关键词
cholesterol; electron microscopy; microdomains; plasma membrane structure;
D O I
10.1073/pnas.0609009103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although much evidence suggests that the plasma membrane of eukaryotic cells is not homogenous, the precise architecture of this important structure has not been clear. Here we use transmission electron microscopy of plasma membrane sheets and specific probes to show that most or all plasma membrane-associated proteins are clustered in cholesterol-enriched domains ("islands") that are separated by "protein-free" and cholesterol-low membrane. These islands are further divided into subregions, as shown by the localization of "raft" and "non-raft" markers to specific areas. Abundant actin staining and inhibitor studies show that these structures are connected to the cytoskeleton and at least partially depend on it for their formation and/or maintenance.
引用
收藏
页码:18992 / 18997
页数:6
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