LBSapSal-vaccinated dogs exhibit increased circulating T-lymphocyte subsets (CD4+ and CD8+) as well as a reduction of parasitism after challenge with Leishmania infantum plus salivary gland of Lutzomyia longipalpis

被引：18

作者：

Aguiar-Soares, Rodrigo Dian de Oliveira ^{[1
]}

Roatt, Bruno Mendes ^{[1
]}

Ker, Henrique Gama ^{[1
]}

Moreira, Nadia Das Dores ^{[1
]}

Mathias, Fernando Augusto Siqueira ^{[1
]}

Cardoso, Jamille Mirelle De Oliveira ^{[1
]}

Gontijo, Nelder Figueiredo ^{[2
]}

Bruna-Romero, Oscar ^{[3
]}

Teixeira-Carvalho, AndrA ^{[4
]}

Martins-Filho, Olindo Assis ^{[4
]}

Correa-Oliveira, Rodrigo ^{[5
]}

Giunchetti, Rodolfo Cordeiro ^{[6
]}

Reis, Alexandre Barbosa ^{[1
,7
,8
]}

机构：

[1] Univ Fed Ouro Preto, Inst Ciencias Exatas Biol, Nucl Pesquisas Ciencias Biol NUPEB, Lab Imunopatol, Ouro Preto, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Lab Fisiol Insetos Hematofagos, Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Belo Horizonte, MG, Brazil

[4] Fundacao Oswaldo Cruz FIOCRUZ, Ctr Pesquisas Rene Rachou, Lab Biomarcadores Diagnost & Monitoracao, Belo Horizonte, MG, Brazil

[5] Fundacao Oswaldo Cruz FIOCRUZ, Centro Pesquisas Rene Rachou, Lab Immunol Celular & Mol, Belo Horizonte, MG, Brazil

[6] Univ Fed Minas Gerais, Dept Morfol, Lab Biol Interacoes Celulares, Belo Horizonte, MG, Brazil

[7] Univ Fed Ouro Preto, Escola Farmacia, Dept Analises Clin, Ouro Preto, MG, Brazil

[8] Tropicais INCT DT, Inst Nacl Ciencia & Technol Doencas, Salvador, BA, Brazil

来源：

PARASITES & VECTORS | 2014年 / 7卷

关键词：

LBSapSal vaccine; Canine visceral leishmaniasis; Immunogenicity; Experimental challenge; Leishmania infantum; Saliva of Lutzomyia longipalpis; CANINE VISCERAL LEISHMANIASIS; SAND FLY SALIVA; LONG-LASTING PROTECTION; NITRIC-OXIDE PRODUCTION; EFFICACY FIELD TRIAL; IMMUNE-RESPONSE; CUTANEOUS LEISHMANIASIS; INFECTED MACROPHAGES; SAPONIN ADJUVANT; VECTOR SALIVA;

D O I：

10.1186/1756-3305-7-61

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程]; 100103 [病原生物学];

摘要：

Background: The development of a protective vaccine against canine visceral leishmaniasis (CVL) is an alternative approach for interrupting the domestic cycle of Leishmania infantum. Given the importance of sand fly salivary proteins as potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in the last few decades. In this context, we previously immunized dogs with a vaccine composed of L. braziliensis antigens plus saponin as the adjuvant and sand fly salivary gland extract (LBSapSal vaccine). This vaccine elicited an increase in both anti-saliva and anti-Leishmania IgG isotypes, higher counts of specific circulating CD8(+) T cells, and high NO production. Methods: We investigated the immunogenicity and protective effect of LBSapSal vaccination after intradermal challenge with 1 x 10(7) late-log-phase L. infantum promastigotes in the presence of sand fly saliva of Lutzomyia longipalpis. The dogs were followed for up to 885 days after challenge. Results: The LBSapSal vaccine presents extensive antigenic diversity with persistent humoral and cellular immune responses, indicating resistance against CVL is triggered by high levels of total IgG and its subtypes (IgG1 and IgG2); expansion of circulating CD5(+), CD4(+), and CD8(+) T lymphocytes and is Leishmania-specific; and reduction of splenic parasite load. Conclusions: These results encourage further study of vaccine strategies addressing Leishmania antigens in combination with proteins present in the saliva of the vector.

引用

页数：10

共 58 条

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