Zero-Mode Waveguide Detection of Flow-Driven DNA Translocation through Nanopores

被引：33

作者：

Auger, Thomas ^{[1
,2
]}

Mathe, Jerome ^{[3
,4
]}

Viasnoff, Virgile ^{[5
,6
]}

Charron, Gaelle ^{[1
,2
]}

Di Meglio, Jean-Marc ^{[1
,2
]}

Auvray, Loic ^{[1
,2
]}

Montel, Fabien ^{[1
,2
]}

机构：

[1] Univ Paris Diderot, F-75205 Paris 13, France

[2] CNRS, UMR 7057, F-75205 Paris 13, France

[3] Univ Evry Val dEssonne, Lab Anal & Modelisat Biol & Environm, F-91025 Evry, France

[4] CNRS, UMR 8587, F-91025 Evry, France

[5] Natl Univ Singapore, Mechanobiol Inst, Singapore 117411, Singapore

[6] CNRS, UMI 3639, Singapore 117411, Singapore

来源：

PHYSICAL REVIEW LETTERS | 2014年 / 113卷 / 02期

关键词：

MEMBRANES; DIFFUSION; INJECTION; MOLECULE; POLYMERS; MOTION;

D O I：

10.1103/PhysRevLett.113.028302

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We directly measure the flow-driven injection of DNA through nanopores at the level of single molecule and single pore using a modified zero-mode waveguide method. We observe a flow threshold independent of the pore radius, the DNA concentration, and length. We demonstrate that the flow injection of DNA in nanopores is controlled by an energy barrier as proposed in the de Gennes-Brochard suction model. Finally, we show that the height of the energy barrier is modulated by functionalizing the nanopores.

引用

页数：5

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