Structure and regulation of the dnaA promoter region in three Streptomyces species

被引：15

作者：

Jakimowicz, D

Majka, J

Lis, B

Konopa, G

Wegrzyn, G

Messer, W

Schrempf, H

Zakrzewska-Czerwinska, J

机构：

[1] Polish Acad Sci, Ludwik Hirszfeld Inst Immunol & Expt Therapy, PL-53114 Wroclaw, Poland

[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[3] Univ Gdansk, Dept Mol Biol, PL-80822 Gdansk, Poland

[4] Univ Osnabruck, Fachbereich Biol Chem, D-49069 Osnabruck, Germany

来源：

MOLECULAR AND GENERAL GENETICS | 2000年 / 262卷 / 06期

关键词：

DnaA box; DnaA protein; promoter; autoregulation;

D O I：

10.1007/PL00008652

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The regulatory region of the Streptomyces dnaA gene comprises a single promoter and two DnaA boxes that are located upstream of the promoter. Comparative analysis of the dnaA promoter region from S. chrysomallus, S. lividans and S. reticuli revealed that the location, spacing and orientation of the DnaA boxes are conserved. In vitro studies demonstrated that efficient binding of the Streptomyces DnaA protein to DNA requires the presence of two DnaA boxes. In vivo analysis of dnaA promoter mutants deleted for one or both DnaA boxes indicated that the dnaA gene is autoregulated. However, the degree of derepression observed is relatively modest.

引用

页码：1093 / 1102

页数：10

共 41 条

[1] AUTO-REGULATION OF THE DNAA GENE OF ESCHERICHIA-COLI-K12 [J].