An improved general approach for cloning and characterizing telomeres:: the protozoan parasite Trypanosoma cruzi as model organism

被引：11

作者：

Chiurillo, MA

Santos, MRM

Da Silveira, JF

Ramírez, JL

机构：

[1] Cent Univ Venezuela, Inst Biol Expt, Lab Genet Mol, Caracas 1041 A, Venezuela

[2] Inst Estudios Avanzados IDEA, Ctr Biotecnol, MCT, Caracas, Venezuela

[3] Escola Paulista Med Unifesp, Dept Inmunol Micro Inmuno & Parasitol, BR-04023062 Sao Paulo, Brazil

来源：

GENE | 2002年 / 294卷 / 1-2期

基金：

巴西圣保罗研究基金会;

关键词：

telomere organization; bacterial artificial chromosome vector-adaptor cloning of telomeres;

D O I：

10.1016/S0378-1119(02)00768-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

We here describe a general strategy for cloning and characterizing telomeric and sub-telomeric regions of the human protozoan parasite Trypanosoma cruzi. The use of a bacterial artificial chromosome vector and a telomeric adaptor produced stable telomeric recombinant clones with inserts ranging from 5 to 25 kb. Analysis of these recombinants provided unique landmarks for chromosomal mapping and sequencing and enabled us to derive a more accurate picture of T cruzi telomeric organization. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：197 / 204

页数：8

相关论文

共 42 条

[1]

The genome sequence of Drosophila melanogaster [J].

Adams, MD ;

Celniker, SE ;

Holt, RA ;

Evans, CA ;

Gocayne, JD ;

Amanatides, PG ;

Scherer, SE ;

Li, PW ;

Hoskins, RA ;

Galle, RF ;

George, RA ;

Lewis, SE ;

Richards, S ;

Ashburner, M ;

Henderson, SN ;

Sutton, GG ;

Wortman, JR ;

Yandell, MD ;

Zhang, Q ;

Chen, LX ;

Brandon, RC ;

Rogers, YHC ;

Blazej, RG ;

Champe, M ;

Pfeiffer, BD ;

Wan, KH ;

Doyle, C ;

Baxter, EG ;

Helt, G ;

Nelson, CR ;

Miklos, GLG ;

Abril, JF ;

Agbayani, A ;

An, HJ ;

Andrews-Pfannkoch, C ;

Baldwin, D ;

Ballew, RM ;

Basu, A ;

Baxendale, J ;

Bayraktaroglu, L ;

Beasley, EM ;

Beeson, KY ;

Benos, PV ;

Berman, BP ;

Bhandari, D ;

Bolshakov, S ;

Borkova, D ;

Botchan, MR ;

Bouck, J ;

Brokstein, P .

SCIENCE, 2000, 287 (5461) :2185-2195

[2]

MOLECULAR KARYOTYPE OF CLONE CL BRENER CHOSEN FOR THE TRYPANOSOMA-CRUZI GENOME PROJECT [J].

CANO, MI ;

GRUBER, A ;

VAZQUEZ, M ;

CORTES, A ;

LEVIN, MJ ;

GONZALEZ, A ;

DEGRAVE, W ;

RONDINELLI, E ;

ZINGALES, B ;

RAMIREZ, JL ;

ALONSO, C ;

REQUENA, JM ;

DASILVEIRA, JF .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1995, 71 (02) :273-278

[3]

Isolation and characterisation of genomic and cDNA clones coding for a serine-, alanine-, and proline-rich protein of Trypanosoma cruzi [J].

Carmo, MS ;

Santos, MRM ;

Cummings, LM ;

Araya, JE ;

Yamauchi, LM ;

Yoshida, N ;

Mortara, RA ;

da Silveira, JF .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2001, 31 (03) :259-264

[4]

Organization of telomeric and sub-telomeric regions of chromosomes from the protozoan parasite Trypanosoma cruzi [J].

Chiurillo, MA ;

Cano, I ;

Da Silveira, JF ;

Ramirez, JL .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1999, 100 (02) :173-183

[5]

Cloning and characterization of Leishmania donovani telomeres [J].

Chiurillo, MA ;

Beck, AE ;

Devos, T ;

Myler, PJ ;

Stuart, K ;

Ramirez, JL .

EXPERIMENTAL PARASITOLOGY, 2000, 94 (04) :248-258

[6]

TRANS-SIALIDASE - A UNIQUE ENZYME-ACTIVITY DISCOVERED IN THE PROTOZOAN TRYPANOSOMA-CRUZI [J].

COLLI, W .

FASEB JOURNAL, 1993, 7 (13) :1257-1264

[7]

THE SURFACE TRANS-SIALIDASE FAMILY OF TRYPANOSOMA-CRUZI [J].

CROSS, GAM ;

TAKLE, GB .

ANNUAL REVIEW OF MICROBIOLOGY, 1993, 47 :385-411

[8]

STRUCTURE AND VARIABILITY OF HUMAN-CHROMOSOME ENDS [J].

DELANGE, T ;

SHIUE, L ;

MYERS, RM ;

COX, DR ;

NAYLOR, SL ;

KILLERY, AM ;

VARMUS, HE .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (02) :518-527

[9]

CHARACTERIZATION OF A SIMPLE, HIGHLY REPETITIVE DNA-SEQUENCE FROM THE PARASITE LEISHMANIA-DONOVANI [J].

ELLIS, J ;

CRAMPTON, J .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 29 (01) :9-17

[10]

Functional diversity in the trans-sialidase and mucin families in Trypanosoma cruzi [J].

Frasch, ACC .

PARASITOLOGY TODAY, 2000, 16 (07) :282-286

← 1 2 3 4 5 →