ELISA and HPLC analysis of ochratoxin A in red wines of Croatia

被引：86

作者：

Flajs, D. ^{[1
]}

Domijan, A-M ^{[1
]}

Ivic, D. ^{[2
]}

Cvjetkovic, B. ^{[2
]}

Peraica, M. ^{[1
]}

机构：

[1] Inst Med Res & Occupat Hlth, Toxicol Unit, Zagreb 10000, Croatia

[2] Univ Zagreb, Fac Agron, Dept Plant Pathol, Zagreb 10000, Croatia

来源：

FOOD CONTROL | 2009年 / 20卷 / 06期

关键词：

Ochratoxin A; ELISA; HPLC; Aspergillus carbonarius; ASPERGILLUS-CARBONARIUS; A PRODUCTION; GRAPES; POPULATION; NIGER; OTA;

D O I：

10.1016/j.foodcont.2008.08.021

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ochratoxin A (OTA) contaminates wine all round the world, and its consumption may significantly increase human exposure to this toxin. In this study, enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) for OTA analysis were tested on must and wine samples collected in Croatia. The results of ELISA and HPLC analysis of OTA in naturally contaminated red wines correlated well (r = 0.821), and the correlation was better at higher OTA concentrations. In contrast to HPLC, ELISA could not detect very low OTA concentrations. OTA concentrations in must (range 19-50 ng/l) were higher than in the wines (range 0-21 ng/l). No must samples showed the presence of Aspergillus carbonarius. which is a common OTA-producing mould affecting grapes. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：590 / 592

页数：3

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