Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response

被引:876
作者
Sunderkötter, C
Nikolic, T
Dillon, MJ
van Rooijen, N
Stehling, M
Drevets, DA
Leenen, PJM
机构
[1] Erasmus MC, Dept Immunol, NL-3015 GE Rotterdam, Netherlands
[2] Univ Munster, Inst Expt Dermatol, D-4400 Munster, Germany
[3] Univ Munster, Dept Dermatol, D-4400 Munster, Germany
[4] Univ Ulm, Dept Dermatol & Allergol, Ulm, Germany
[5] Univ Oklahoma, Hlth Sci Ctr, Infect Dis Sect, Oklahoma City, OK 73014 USA
[6] Vet Affairs Med Ctr, Oklahoma City, OK 73014 USA
[7] Free Univ Amsterdam, Med Ctr, Dept Mol Cell Biol, Amsterdam, Netherlands
关键词
D O I
10.4049/jimmunol.172.7.4410
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Blood monocytes are well-characterized precursors for macrophages and dendritic cells. Subsets of human monocytes with differential representation in various disease states are well known. In contrast, mouse monocyte subsets have been characterized minimally. In this study we identify three subpopulations of mouse monocytes that can be distinguished by differential expression of Ly-6C, CD43, CD11c, MBR, and CD62L. The subsets share the characteristics of extensive phagocytosis, similar expression of M-CSF receptor (CD115), and development into macrophages upon M-CSF stimulation. By eliminating blood monocytes with dichloromethylene-bisphosphonate-loaded liposomes and monitoring their repopulation, we showed a developmental relationship between the subsets. Monocytes were maximally depleted 18 h after liposome application and subsequently reappeared in the circulation. These cells were exclusively of the Ly-6C(high) subset, resembling bone marrow monocytes. Serial flow cytometric analyses of newly released Ly-6C(high) monocytes showed that Ly-6C expression on these cells was down-regulated while in circulation. Under inflammatory conditions elicited either by acute infection with Listeria monocytogenes or chronic infection with Leishmania major, there was a significant increase in immature Ly-6C(high) monocytes, resembling the inflammatory left shift of granulocytes. In addition, acute peritoneal inflammation recruited preferentially Ly-6C(med-high) monocytes. Taken together, these data identify distinct subpopulations of mouse blood monocytes that differ in maturation stage and capacity to become recruited to inflammatory sites.
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页码:4410 / 4417
页数:8
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