A multicenter investigation with D-FISH BCR/ABL1 probes

被引：24

作者：

Dewald, G

Stallard, R

Alsaadi, A

Arnold, S

Blough, R

Ceperich, TM

Elejalde, BR

Fink, J

Higgins, JV

Higgins, RR

Hoeltge, GA

Hsu, WT

Johnson, EB

Kronberger, D

McCorquodale, DJ

Meisner, LF

Micale, MA

Oseth, L

Payne, JS

Schwartz, S

Sheldon, S

Sophian, A

Storto, P

Van Tuinen, P

Wenger, GD

Wiktor, A

Willis, LA

Yung, JF

Zenger-Hain, J

机构：

[1] Mayo Clin & Mayo Fdn, Cytogenet Lab, Rochester, MN 55905 USA

[2] Univ Wisconsin, Great Lakes Reg Genet Grp, Madison, WI USA

[3] William Beaumont Hosp, Royal Oak, MI 48072 USA

[4] Childrens Hosp, Med Ctr, Akron, OH USA

[5] Childrens Hosp, Cincinnati, OH 45229 USA

[6] Indiana Univ, Indianapolis, IN 46204 USA

[7] Med Genet Inst SC, Brown Deer, WI USA

[8] Hennepin Cty Med Ctr, Minneapolis, MN 55415 USA

[9] Spectrum Hlth, Grand Rapids, MI USA

[10] Cleveland Clin Fdn, Cleveland, OH 44195 USA

[11] Rush Presbyterian St Lukes Med Ctr, Chicago, IL 60612 USA

[12] Univ Wisconsin, Madison, WI USA

[13] Marshfield Labs, Marshfield, WI USA

[14] Michael Reese Hosp & Med Ctr, Chicago, IL 60616 USA

[15] Univ Wisconsin, State Lab Hyg, Madison, WI USA

[16] Med Coll Ohio, Toledo, OH 43699 USA

[17] Fairview Univ Med Ctr, Minneapolis, MN USA

[18] Rockford Mem Hosp, Rockford, IL USA

[19] Case Western Reserve Univ, Cleveland, OH 44106 USA

[20] Univ Hosp Cleveland, Cleveland, OH 44106 USA

[21] Univ Michigan, Ann Arbor, MI 48109 USA

[22] Lutheran Gen Hosp, Park Ridge, IL USA

[23] Michigan State Univ, E Lansing, MI 48824 USA

[24] Med Coll Wisconsin, Milwaukee, WI 53226 USA

[25] Childrens Hosp, Columbus, OH 43205 USA

[26] Henry Ford Med Grp, Detroit, MI USA

[27] Childrens Med Ctr, Dayton, OH USA

[28] Mercy Hosp & Med Ctr, Chicago, IL USA

[29] Oakwood Hosp, Dearborn, MI USA

来源：

CANCER GENETICS AND CYTOGENETICS | 2000年 / 116卷 / 02期

关键词：

D O I：

10.1016/S0165-4608(99)00120-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Twenty-eight laboratories evaluated a new fluorescence in situ hybridization (FISH) strategy for chronic myeloid leukemia. In a three-part study: bcr/abl1 D-FISH probes were used to study bone marrow specimens. First, laboratories familiarized themselves with the strategy by applying it to known normal and abnormal specimens. Then, collectively the laboratories studied 20 normal and 20 abnormal specimens blindly and measured workload. Finally each laboratory and two experts studied six serial dilutions with 98-0%;, abnormal nuclei. Using the reported normal cutoff of <1% abnormal nuclei, participants reported no false-negative cases and 15 false-positive cases (1-6.6% abnormal nuclei). Results provided by participants for serial dilutions approximated the expected percentages of abnormal nuclei, but those from the experts exhibited greater precision. The clinical sensitivity, precision, nomenclature, workload, recommendations for training and quality assurance in methods using D-FISH in clinical practice are discussed. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：97 / 104

页数：8

共 14 条

[1] A special fluorescent in situ hybridization technique to study peripheral blood and assess the effectiveness of interferon therapy in chronic myeloid leukemia [J].