Activity and adsorption of lipase from Nigella sativa seeds on Celite at different pH values

被引：27

作者：

Akova, A ^{[1
]}

Ustun, G ^{[1
]}

机构：

[1] Istanbul Tech Univ, Dept Chem Engn, TR-80626 Istanbul, Turkey

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 05期

关键词：

activity; adsorption; Celite; immobilization; lipase; Nigella sativa;

D O I：

10.1023/A:1005668500716

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase from Nigella sativa seeds was immobilized by adsorption on Celite 535 from phosphate buffer solutions varying pH values of 5.0-8.0 at 25 degrees C. Langmuir isotherms described the adsorption equilibria well for lipase adsorption at all pH range. The saturation capacity for adsorption of lipase increased from 14.5 to 24.3 mg g(-1) Celite as the adsorption pH was reduced from 8 to 5, but the adsorption equilibrium constant remained constant and was determined to be 1.92 x 10(5) M-1. The adsorbed enzymes showed different activity values depending on the pH of the adsorption medium. The immobilized enzymes prepared at pH 6 displayed the highest activity values.

引用

页码：355 / 359

页数：5

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