Calcium-induced Cytochrome c release from rat brain mitochondria is altered by digitonin

被引：20

作者：

Brustovetsky, N

Jemmerson, R

Dubinsky, JM

机构：

[1] Univ Minnesota, Dept Neurosci, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA

来源：

NEUROSCIENCE LETTERS | 2002年 / 332卷 / 02期

关键词：

digitonin; permeability transition; Cytochrome c; apoptosis; brain mitochondria; calcium;

D O I：

10.1016/S0304-3940(02)00948-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To determine if calcium could release Cytochrome c (Cyt c) from brain mitochondria without activating the permeability transition (mPT), brain mitochondria were prepared in two different ways. Digitonin was used to lyse synaptosomes and release synaptosomal mitochondria or a Percoll gradient was used to separate non-synaptosomal mitochondria from the synaptosomes. In gradient-purified mitochondria, low levels of added digitonin produced swelling and Cyt c release. Digitonin augmented Ca2+-induced Cyt c release that was insensitive to the mPT inhibitors, cyclosporin A CsA and ADP. Similarly, in mitochondria prepared with digitonin, these inhibitors also failed to prevent Ca2+-induced Cyt c release. Thus the mPT-independent, Ca2+-induced Cyt c release pathway was attributable to alteration of the permeability properties of the outer mitochondrial membrane by digitonin. (C) 2002 Published by Elsevier Science Ireland Ltd.

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收藏

页码：91 / 94

页数：4

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