Phenotypical and molecular characterization of the Tomato mottle Taino virus-Nicotiana megalosiphon interaction

被引：8

作者：

Collazo, Cyrelys

Ramos, Pedro Luis

Chacon, Osmany

Borroto, Carlos Javier

Lopez, Yunior

Pujol, Merardo

Thomma, Bart P. H. J.

Hein, Ingo

Borras-Hidalgo, Orlando

机构：

[1] Plant Funct Genom, Ctr Genet Engn & Biotechnol, Havana 10600, Cuba

[2] Tobacco Res Inst, Havana, Cuba

[3] Wageningen Univ, Lab Phytopathol, NL-6709 PD Wageningen, Netherlands

[4] Scottish Crop Res Inst, Dundee DD25DA, Scotland

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2005年 / 67卷 / 3-5期

关键词：

plant defense; ToMoTV; Nicotiana megalosiphon; suppression subtractive hybridization; SSH; Begomovirus;

D O I：

10.1016/j.pmpp.2006.02.003

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Tomato mottle Taino virus (ToMoTV) infection causes significant yield losses in plants of Various Solanaceous species. In this Study, the interaction between Nicotiana megalosiphon and ToMoTV was characterized on a phenotypical and Molecular basis. In order to isolate genes that are differentially expressed during the interaction of N. megalosiphon with ToMoTV, a PCR-based suppression subtractive hybridization (SSH) was utilized. RNA dot-blot analysis confirmed induction of representative genes upon ToMoTV inoculation at different time points. Interestingly, most of the genes identified are reported here for the first time to be involved in the response of M megalosiphon to begomovirus infection. (c) 2006 Elsevier Ltd. All rights reserved.

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收藏

页码：231 / 236

页数：6

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